Kimberly K. Buhman, Ph.D. Assistant Professor Foods and Nutrition
Purdue University Stone Hall Room 209 700 West State Street West Lafayette, IN 47907-2059 Phone: (765)496-6872 Email: kbuhman@purdue.edu
EDUCATION BACKGROUND
B.S. in Agricultural Biochemistry at Iowa State University in 1992
Ph.D. in Nutritional Biochemistry at Purdue University in 1999
Post-Doc. in Lipid Metabolism at Gladstone Institute of Cardiovascular Disease in 2002
Post-Doc. in Cardiology at Washington University School of Medicine in 2005
DISCOVERY
Research in Dr. Buhman’s laboratory focuses on the process of intestinal lipid absorption and how it regulates whole animal energy balance. Current research in the laboratory addresses the following questions: 1) What is the role of triglyceride synthesis in absorptive cells of the intestine on whole animal energy balance? 2) What is the role of fatty acid catabolism in absorptive cells of the intestine on whole animal energy balance? 3) What is the role of triglyceride storage in lipid droplets during the process of intestinal lipid absorption? Discovery Publications (selected)
Zhu, J., Lee, B., Buhman, K.K., Cheng, J.X. (2009) Direct Observation of a Dynamic Triglyceride Pool in Mouse Enterocytes during Dietary Fat Absorption by Coherent Anti-Stokes Raman Scattering Imaging. J. of Lipid Research. epub ahead of print
Antalis, C.J., Arnold, T., Lee, B-G, Buhman K.K., and Siddiqui, R.A. (2009) Docosahexaenoic acid is a substrate for ACAT1 and inhibits cholesterol ester formation from oleic acid in MCF-10A cells. Prostaglandins, Leukotrienes, and Essential Fatty Acids. epub ahead of print
Siddiqui, S.M.K., Chang, E., Li, J., Burlage, C., Zhou, M., Buhman, K.K., Koser, S., Donkin, S.S., Teegarden, D. (2008) Dietary Intervention with Vitamin D, Calcium and Whey Protein Reduced Fat Mass and Increased Lean Mass in Rats. Nutrition Research. 28(11):783-90.
Borradaile N.M., Buhman K.K., Listenberger L.L., Magee C.J., Morimoto E.T.A., Ory D.S., Schaffer J.E. (2006) A critical role for eukaryotic elongation factor (eEF) 1A-1 in lipotoxic cell death. Mol Biol Cell. 17(2):770-8.
Tang, Y, Swietlicki E. A., Jiang, S., Buhman, K.K., Davidson, N.O., Burkly, L.C., Levin M.S., Rubin, D. C. (2006) Increased apoptosis and accelerated epithelial migration following inhibition of hedgehog signaling in adaptive small bowel post-resection. Am J. Physiol. Gastrointest. Liver Physiol. 290(6):G1280-1288.
Repa J.J., Buhman K.K., Farese R.V. Jr, Dietschy J.M., Turley S.D. (2004) ACAT2 deficiency limits cholesterol absorption in the cholesterol-fed mouse: Impact on hepatic cholesterol homeostasis. Hepatology 40(5):1088-1097.
Buhman K.K., Wang L.C., Tang Y., Swietlicki E.A., Kennedy, S., Xie, Y., Liu, Z.Y., Burkly L.C., Levin M.S., Rubin D.C., and Davidson N.O. (2004) Inhibition of Hedgehog signaling protects adult mice from diet-induced weight gain. J. Nutrition. 134(11):2979-84.
Wilner E.L., Tow B., Buhman K.K., Wilson M., Sanan D.A., Rudel L.L., Farese R.V. Jr. (2003) Deficiency of acyl CoA:cholesterol acyltransferase 2 prevents atherosclerosis in apolipoprotein E-deficient mice. Proc. Natl. Acad. Sci. U S A. 100(3):1262-7.
Buhman K.K., Smith S.J., Stone S.J., Repa J.J., Wong J.S., Knapp F.F. Jr, Burri B.J., Hamilton R. L., Abumrad N.A., Farese R.V. Jr. (2002) DGAT1 is not essential for intestinal triacylglycerol absorption or chylomicron synthesis. J. Biol. Chem. 277(28):25474-9.
Buhman, K.K., M. Accad, S. Novak, R.S. Choi, J.S. Wong, R.L. Hamilton, S. Turley, and R.V. Farese, Jr. (2000) Resistance to diet-induced hypercholesterolemia and gallstone formation in ACAT2-deficient mice. Nat Med. 12:1341-7.
Books, Chapters, and Monographs Publications
Buhman K.K., H.C. Chen, R.V. Farese (2001) The enzymes of neutral lipid synthesis.
J. Biol. Chem. 276(44):40369-72.
Macronutrient Metabolism in Health and Disease (FN43700) - Metabolism of the macronutrients, carbohydrate, lipid and protein in humans integrating physiology, biochemistry, and nutrition with a focus on maintaining optimal health and preventing disease.
Introduction to Honors Research (FN29700) - Introduces students to the design and implementation of an honors research project.
Lipid Regulation and Cell Function (FN59000) - Introduction of hot topics in the area of lipid regualtion and cell function and critical evaluation of scientific literature.
Nutritional Physiology and Biochemistry III (FN60700) - Integration of biochemical and physiological functions of nutrients in humans and animals, emphasizing transport and metabolism in the context of cardiovascular function. Dr. Buhman teaches a section related to lipoprotein metabolism.
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Contact Information
Foods and Nutrition Purdue University 700 W. State Street West Lafayette, IN 47907-2059
